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명아주 추출물의 항산화 효과에 의한 배양 인체피부섬유모세포에 대한 카드뮴 손상방어 |
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손영우(Young Woo Son), 최유선(Yu Sun Choi) |
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| Abstract |
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To evaluate the cytotoxicity of cadmium(Cd), an environmental pollutant, and the protective effect of Chenopodium album var. centrorubrum(CAC) extract on the Cd-induced cytotoxicity in cultured human skin fibroblasts(Detroit 551). Cultured Detroit 551 cells were treated or pretreated with cadmiun chloride(CdCl₂) or CAC extract. In this study, CdCl₂ significantly decreased cell viability compared with control, and XTT₅₀ or XTT₅₀ value(midcytotoxicity value, MCV) was determined at a concentration of 41.1μM or 43.1μM. The MCV of CdCl₂ was highly toxic by the criteria of Borenfreund and Puerner. While, CdCl₂-induced cytotoxicity was prevented by superoxide dismutase(SOD), an antioxidative enzyme. In the protective effect of CAC extract on CdCl₂-induced cytotoxicity, CAC extract prevented CdCl₂-induced cytotoxicity by the significant increase of cell viability decreased by CdCl₂-induced cytotoxicity. And also, CAC extract showed antioxidative effects such as electron donating activity(EDA) and inhibitory activity of lipid peroxidation(LP). From above the results, it is suggested that CdCl₂ was highly toxic to cultured Detroit 551 cells and CAC extract was effective in the blocking CdCl₂-induced cytotoxicity by the antioxidative effects such as EDA and inhibitory activity of LP. In conclusion, the physical activity of natural component such as CAC extract may be a useful resource for preventing diseases mediated by reactive oxygen species(ROS) such as heavy metal toxication, stroke or dementia. |
| KeyWords:
Lipid peroxidation, Midcytotoxicity value, Physical activity, Superoxide dismutase |
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