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Glucose Oxidase로 손상된 배양 C6 Glioma 세포에 대한 인동덩굴 추출물의 항산화 효과 |
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박선희(Sun Hee Park), 강민정(Min Jung Kwang), 이영찬(Young Chan Lee), 이재규(Jai Kyoo Lee) |
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| Abstract |
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To evaluate the protective effect of Lonicera japonica Thunb.(LJ) extract on glucose oxidase(GO)-mediated cytotoxicity in cultured C6 glioma cells, and the antioxidative effect of LJ extract, colorimetric assays such as the cell viability, DPPH-radical scavenging activity, superoxide dismutase(SOD)-like activity, lipid peroxidation(LP) inhibitory activity and lactate dehydrogenase(LDH) activity were performed. For this study, C6 glioma cells were treated with the media containing 15~35 mUㆍmL⁻¹ GO for 4 hours, respectively. In this study, GO significantly decreased cell viability in dose-dependent manner, and the XTT₅₀ value was measured at 40 mUㆍmL⁻¹ GO. In the effect of LJ extract on GO-mediated cytotoxicity, LJ extract significantly increased the cell viability which was decreased by GO. In the antioxidative effects, LJ extract showed the DPPH-radical scavenging activity, SOD-like activity, the inhibitory activity of LP and the decrease of LDH activity. From these results, it is suggested that the GO showed cytotoxicity on cultured C6 glioma cells by the decrease of cell viability, and LJ extract was effective in the protection of GO-mediated cell injury by antioxidative effect. |
| KeyWords:
Cell viability, LDH activity, SOD-like activity |
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