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J. Korean Soc. People Plants Environ. 2012;15(3):155-161. Published online June 30, 2012.
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- Abstract
- To evaluate the cytotoxicity of cadmium(Cd), an environmental pollutant, and the protective effect of Chenopodium album var. centrorubrum(CAC) extract on the Cd-induced cytotoxicity in cultured human skin fibroblasts(Detroit 551). Cultured Detroit 551 cells were treated or pretreated with cadmiun chloride(CdCl₂) or CAC extract. In this study, CdCl₂ significantly decreased cell viability compared with control, and XTT₅₀ or XTT₅₀ value(midcytotoxicity value, MCV) was determined at a concentration of 41.1μM or 43.1μM. The MCV of CdCl₂ was highly toxic by the criteria of Borenfreund and Puerner. While, CdCl₂-induced cytotoxicity was prevented by superoxide dismutase(SOD), an antioxidative enzyme. In the protective effect of CAC extract on CdCl₂-induced cytotoxicity, CAC extract prevented CdCl₂-induced cytotoxicity by the significant increase of cell viability decreased by CdCl₂-induced cytotoxicity. And also, CAC extract showed antioxidative effects such as electron donating activity(EDA) and inhibitory activity of lipid peroxidation(LP). From above the results, it is suggested that CdCl₂ was highly toxic to cultured Detroit 551 cells and CAC extract was effective in the blocking CdCl₂-induced cytotoxicity by the antioxidative effects such as EDA and inhibitory activity of LP. In conclusion, the physical activity of natural component such as CAC extract may be a useful resource for preventing diseases mediated by reactive oxygen species(ROS) such as heavy metal toxication, stroke or dementia.
Keywords :Lipid peroxidation;Midcytotoxicity value;Physical activity;Superoxide dismutase
